Molecular cloning : a laboratory manual. Volume 3 /

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Main Author: Green, Michael R.
Other Authors: Sambrook, Joseph
Format: Book
Language:English
Published: Cold Spring Harbor : Cold Spring Harbor Laboratory Press, c2012.
Edition:4th ed.
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Table of Contents:
  • Analysis of gene regulation using reporter systems
  • RNA interference and small RNA analysis
  • Expressing cloned genes for protein production, purification, and analysis
  • Cross-linking technologies for analysis of chromatin structure and function
  • Mapping of in vivo RNA-bindings sites by UV-cross-linking immunoprecipitation (CLIP)
  • Gateway-compatible yeast one-hybrid and two-hybrid assays
  • Appendix 1: reagents and buffers
  • Appendix 2: commonly used techniques
  • Appendix 3: detection systems
  • Appendix 4: general safety and hazardous material